High Springs is a city in Alachua County, Florida, United States. It is the fourth-largest city in Alachua County and seventh-largest in North Ce…High Springs is a city in Alachua County, Florida, United States. It is the fourth-largest city in Alachua County and seventh-largest in North Central Florida. The population was 6,215 at the 2020 census, up from 5,350 at the 2010 census. It is part of the Gainesville, Florida Metropolitan Statistical Area.