Melting curve analysis is based on a robust post-PCR biophysical measurement and therefore has advantages over other mutation-detection methods that derive information from the amplification ...

Validation of PCR thermal cycler performance is crucial in order to obtain reliable results. By applying high resolution melting curve (HRM) analysis using a defined PCR amplicon and EvaGreen dye, ...

Multiplex PCR and post-amplification melting curve analysis. Rapid-cycle PCR amplification was performed with Nras primers and probes for codons 12 and 13, and codon 61 as summarized in Table 3.

To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right). MeltPlex®utilizes a system of labelled ...

Introduction: Receptor-mediated estrogen activation participates in the development and progression of breast cancer. Estrogen receptor (ER)-α polymorphism has been found to be associated with ...

After PCR, a post-PCR melting curve is performed, in which the temperature is gradually increased. The temperature at which 50 percent of the SMB and the viral gene target are attached is called ...

Dr. Zhou noted that the strategy of one-step nested asymmetric PCR melting analysis overcomes the bottlenecks of high homology and GC-rich secondary structure that limited previous types of analyses.

Melt curve analysis suitability: Because Scorpions primers generate fluorescence under non-hydrolytic conditions by target hybridisation, post-PCR melt curve analysis can be performed; ...

Seegene Inc. (096530.KQ), a leading developer of multiplex PCR technologies, ... Even melt curve analysis after amplification has many drawbacks, ...

December 1, 2007 (Vol. 27, No. 21) Using the LightCycler 480 System for Discovery and Analysis of Genetic Variation LightCycler® Real-time PCR Systems from Roche (www.roche.com) are efficient ...