In digital PCR (dPCR) analysis ... To this end, DQ24 offers a new possibility, which is to use melting curve analysis to achieve multiple detection targets in a single-color assay.

COLD-PCR exploits a small but critical difference in amplicon melting temperature. COLD: Coamplification at lower denaturation temperature; WT: Wild-type. Data taken from [24,35]. High-resolution ...