In digital PCR (dPCR) analysis ... To this end, DQ24 offers a new possibility, which is to use melting curve analysis to achieve multiple detection targets in a single-color assay.

The experiments were performed with the QuantStudio™ 5 Real-Time PCR System (Thermofisher Scientific) and the qPCR conditions followed those recommended by the kit manufacturer. The standard curve was ...

The SALSA MC002 SMA Newborn Screen uses PCR amplification and melt curve analysis to determine the presence or absence of exon 7 in the SMN1 and SMN2 genes from a dried blood spot sample.

Real-time PCR was performed with a LightCycler 480 (Life Technologies ... The identity and purity of the amplified product were assessed by analysing the melting curve, which was performed at the end ...

COLD-PCR exploits a small but critical difference in amplicon melting temperature. COLD: Coamplification at lower denaturation temperature; WT: Wild-type. Data taken from [24,35]. High-resolution ...

and analyzed either in real time or via most downstream molecular applications. COLD-PCR exploits a small but critical difference in amplicon melting temperature.

Recently, the work was completed by the group of Prof. Sheng Meng from the Institute of Physics, Chinese Academy of Sciences, and Beijing National Research Center for Condensed Matter Physics. By ...

Their findings, published in Physical Review Letters, shed new light on the melting curve and structural phase of iron under extreme conditions. "The goal of this study was to explore the ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...