The experiments were performed with the QuantStudio™ 5 Real-Time PCR System (Thermofisher Scientific) and the qPCR conditions followed those recommended by the kit manufacturer. The standard curve was ...

The only reason they were able to make this discovery is because of the melting ice patch. "I'm thrilled because it's a window on the past. It tells us what this high-elevation environment was ...

UPDATE: Real Women Have Curves: The Musical has announced additional members of the production’s Broadway company who will join the previously announced Tatianna Córdoba (Ana) and Justina ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

Blue puddles of water were visible atop the Amery Ice Shelf in East Antarctica after periods of record melting. Satellite data from early 2025 revealed extensive melting at Antarctica’s Amery Ice ...

Their findings, published in Physical Review Letters, shed new light on the melting curve and structural phase of iron under extreme conditions. "The goal of this study was to explore the ...

a post-PCR melt curve is possible. Just like standard dual-labeled BHQ Probes it is possible to multiplex assays using Molecular Beacons. Multiplexing allows for the detection of several targets ...

PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification ...

PCR-based detection of variant sequences is often carried ... The signal of the whole set of probes during the melting curve analysis can be used to create an annealing curve that shows the ...

Dried blood spots were blinded prior to DNA extraction and analysis by triplet primed PCR (TP-PCR) and melt curve analysis (MCA). All expansion-positive and representative expansion-negative samples ...