Roche Diagnostics and Takara Bio, the Shiga (Japan)-based supplier of systems for use in life science research, announced that they have entered into a license agreement for use of the melting curve ...

Melting curve analysis is based on a robust post-PCR biophysical measurement and therefore has advantages over other mutation-detection methods that derive information from the amplification ...

During melting curve analysis, the temperature of the PCR product(s) is gradually raised until the double-stranded DNA (dsDNA) begins to dissociate.

Multiplex PCR and post-amplification melting curve analysis. Rapid-cycle PCR amplification was performed with Nras primers and probes for codons 12 and 13, and codon 61 as summarized in Table 3.

Seegene Inc. (096530.KQ), a leading developer of multiplex PCR technologies, ... Even melt curve analysis after amplification has many drawbacks, ...

Dr. Zhou noted that the strategy of one-step nested asymmetric PCR melting analysis overcomes the bottlenecks of high homology and GC-rich secondary structure that limited previous types of analyses.

After PCR, a post-PCR melting curve is performed, in which the temperature is gradually increased. The temperature at which 50 percent of the SMB and the viral gene target are attached is called ...

Melt curve analysis suitability: Because Scorpions primers generate fluorescence under non-hydrolytic conditions by target hybridisation, post-PCR melt curve analysis can be performed; ...

Introduction: Receptor-mediated estrogen activation participates in the development and progression of breast cancer. Estrogen receptor (ER)-α polymorphism has been found to be associated with ...