In this study researchers from Charité-Universitätsmedizin Berlin present high resolution melting curve analysis as a novel validation method for real-time PCR instruments.

Melting curve analysis is based on a robust post-PCR biophysical measurement and therefore has advantages over other mutation-detection methods that derive information from the amplification ...

The melting temperature (Tm) of a PCR product is dependent on both the length and sequence of the DNA. Melting curve analysis has traditionally been used to identify nonspecific products or ...

By utilizing melting curve readout of modified probes –one for each target -rather than the only amplicons, the system adds an extra level of specificity to melting curve analysis. Reaction and ...

PCR single-strand conformation polymorphism technology was employed and real-time PCR melting curve analysis was performed.

Dr. Zhou noted that the strategy of one-step nested asymmetric PCR melting analysis overcomes the bottlenecks of high homology and GC-rich secondary structure that limited previous types of analyses.

After PCR, a post-PCR melting curve is performed, in which the temperature is gradually increased. The temperature at which 50 percent of the SMB and the viral gene target are attached is called ...

Melt curve analysis suitability: Because Scorpions primers generate fluorescence under non-hydrolytic conditions by target hybridisation, post-PCR melt curve analysis can be performed ...

Even melt curve analysis after amplification has many drawbacks, such as end-point analysis after amplification, extended turnaround time, lack of quantification and melt temperature variation due ...